Human CILP2 (Cartilage intermediate layer protein 2) QuickTest ELISA Kit Packaging:96 wells Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Human ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

And then biotinylated Mouse IL13RA1 Antibody is added and binds to IL13RA1 in the sample

The plate has been pre-coated with Mouse NEIL3 antibody

You can calculate the concentration of Mouse IL-6 in the samples by comparing the OD of the samples to the standard curve

Then Streptavidin-HRP is added and binds to the Biotinylated FZD1 antibody

Human CILP2 (Cartilage intermediate layer protein 2) QuickTest ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human CILP2 (Cartilage intermediate layer protein 2) QuickTest ELISA Kit measures CILP2 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and