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The concentration of Human FSP in the samples is then determined by comparing the OD of the samples to the standard curve
GABA in the sample or standard competes with a fixed amount of GABA on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to GABA
And then biotinylated Human SERPINA11 Antibody is added and binds to SERPINA11 in the sample
Substrate solution is then added and color develops in proportion to the amount of Mouse DKK1
Then Streptavidin-HRP is added and binds to the Biotinylated CTSC antibody
Human CGA (Chromogranin A) ELISA Kit Packaging:96 wells The concentration of Human FSPThe Human CGA (Chromogranin A) ELISA Kit measures CGA in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CGA antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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